220 highway were blockaded as of Tuesday morning, federal highway police told The New York Times.
On Tuesday, Bolsonaro supporters partially blocked the highway leading to the Brazil's largest airport in São Paulo, forcing the cancellation of at least two dozen flights, according to The New York Times.
Bolsonaro lost Sunday's presidential election to former president Luiz Inácio Lula da Silva in a remarkably close race.
Da Silva, also known as Lula, received 50.83% of the votes, compared to Bolsonaro's 49.17%.
For months, Bolsonaro pushed baseless claims that the election, which many observers expected him to lose, was being tainted by fraud.